DESCRIPTION

• Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com
• Uniprot: Q9Y4K3
• Shiptemp: -80°C (dry ice)
• Synonyms: TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6
• Warnings: Avoid freeze/thaw cycles
• Category: Ubiquitin/Assay kit
• Background: Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications that regulates protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2ˆ¼Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2ˆ¼Ub to the substrate, leading to its mono- or poly-ubiquitination.
• Description: The TRAF6 intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure TRAF6 auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium-labeled ubiquitin (Ub) donor as well as Cy5-labeled Ub acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on TRAF6, this assay measures poly-ubiquitination. As a homogeneous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses.
• Methodology: TR-FRET
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: • Screen molecules that inhibit TRAF6 Ub ligase activity in drug discovery HTS applications • Determine compound IC50 • Perform TRAF6 real-time kinetics
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 78301, 78302, 78303, 78305
• Contraindications: The TRAF6 intrachain TR-FRET Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 5% DMSO solution in buffer and using 4 µl per well.
• Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. 
• Scientific Category: Ubiquitination