Anti-iNOS (Tyr-1055) [conserved site], Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: NOS2
Host: Rabbit
Rrid: AB_10705206
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: iNOS (Tyr-1055)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Nos2 nitric oxide synthase 2, inducible, macrophage, NOS, type II, NOSII, Hepatocyte
Clonality: Rabbit Polyclonal
Immunogen: Phospho-iNOS (Tyr-1055) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Tyr-1055 in human iNOS. This sequence is well conserved in rat and mouse iNOS, and has some homology to the conserved sites in eNOS (Tyr-1087) and nNOS (Tyr-1326).
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 130 kDa* protein corresponding to induced NOS on SDS-PAGE immunoblots of mouse macrophages treated with LPS followed by pervanadate. The antibody also detects a 150 kDa doublet that corresponds with higher molecular weight forms of NOS, which are also observed using other anti-iNOS antibodies. In addition, the antibody detects a non-specific 120 kDa band that has not been identified.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 130
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:400
Species Reactivity: Human, Mouse, and Rat
Target Description: Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes, inducible NOS (iNOS, NOS-II), neuronal NOS (bNOS, NOS-I), and endothelial NOS (eNOS, ecNOS, NOS-III). The inducible form of NOS, iNOS, is Ca2+ independent and is expressed in a broad range of cell types in response to stimulation with cytokines and exposure to microbial products. Phosphorylation of iNOS may regulate its activity and stability. Src kinase-induced phosphorylation of Tyr-1055 in iNOS reduces proteasomal degradation of iNOS, leading to increased NO production. Thus, phosphorylation may be an additional control for regulating iNOS activity in response to inflammatory conditions.
Uniprot Number Immunogen Species: P35228
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Tyr-1055