DESCRIPTION

• Category: REAGENTS
• Keywords: Taq, DNA, Polymerase, Blue, Master, Master Mix, 2.0X, Buffer I, PCR, Hot Start, Apex, 2X
• Paragraph 1: Apex Hot Start Master Mix BLUE is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Apex Hot Start DNA Polymerase, ammonium buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix with Apex Buffer I. Each reaction requires 25uL of the 2.0X reaction mix.
• Paragraph 2: For most standard applications Master Mix BLUE based on Ammonium Buffer I is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix BLUE with Buffer II is the best option.
• Bullet Point 1: Apex Hot Start Master Mix, Blue, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, high yield and high specificity
• Bullet Point 2: Optimized Mix - just add template, primers and water, and conveniently set up reactions at room temperature
• Bullet Point 3: Includes chemically modified hot start version of Apex Taq DNA Polymerase - promotes increased sensitivity, specificity and product yield
• Bullet Point 4: Diminishes the formation of non-specific priming events - suitable for low abundance targets, screening, GC-rich, multiplex and direct colony PCR
• Bullet Point 5: Reliably processes up to 5kb with high product yield, exhibits 5'to 3 exonuclease activity and leaves a 3'dA overhang
• Bullet Point 6: Same as clear version, but with an inert blue dye and stabilizer that allows direct-to-gel analysis after amplification and easy pipetting
• Bullet Point 7: Dye front runs at 100-500 bp (0.5 -1.5 % agarose gel). PCR products can be used for sequencing after PCR Clean-up
• Bullet Point 8: Multiple 1.25ml aliquot packaging prevent repetitive freeze-thaw cycles - mix is stable refrigerated (4ºC) for at least 6 months without risk
• Manufacturer Sku: 42-143