• Form: Antigen Affinity Purified from Pooled Serum
• Gene: BECN1
• Host: Rabbit
• Rrid: AB_2492048
• Type: Primary Antibody
• Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Target: Beclin1 (Ser295)
• Isotype: IgG
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Shipping: Blue Ice
• Synonyms: PG6 antibody, ATG 6 antibody, ATG6 antibody, ATG6 autophagy related 6 homolog antibody, Bcl-2-interacting protein beclin antibody, Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody, Beclin 1 autophagy related antibody, Beclin-1 antibody, Beclin1 antibody, BECN 1 antibody, Becn1 antibody, BECN1_HUMAN antibody, Coiled coil myosin like BCL2 interacting protein antibody, Coiled-coil myosin-like BCL2-interacting protein antibody, GT197 antibody, Protein GT197 antibody, VPS 30 antibody, VPS30 antibody
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of human beclin-1, conjugated to keyhole limpet hemocyanin (KLH).
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~60 kDa beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant.
• Applications: WB
• Ncbi Gene Id: 8678
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 60
• Production Notes: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Dilution Range Wb: 1:250
• Immunogen Species: Human
• Species Reactivity: Human, Mouse, and Rat
• Target Description: Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser-295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).
• Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish
• Uniprot Number Immunogen Species: Q14457
• Post Translational Modification Type: Phosphorylated
• Post Translational Modification Residue: Ser295

DESCRIPTION

PROMOTION TERMS