DESCRIPTION

• Aa: 51-159
• Mw: 39 kDa
• Tags: N-terminal GST-Tag
• Format: Aqueous buffer solution.
• Purity: ≥90%
• Genbank: NM_145803
• Species: Human
• Uniprot: Q9Y4K3
• Shiptemp: -80°C (dry ice)
• Synonyms: TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6, RNF85
• Category: Ubiquitination/Protein
• Constructs: TRAF6 (GST-Pre-51-159)
• Description: Human Recombinant TRAF6 (TNF receptor associated factor 6), encompassing amino acids 51-159. This construct contains an N-terminal GST-tag followed by a PreScission Site to facilitate removal of the GST-tag.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and variable Glutathione
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: E. coli
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: TRAF6 activity was measured using varying amounts of TRAF6 and incubated with E1, E2, and ATP in the presence of an "intrachain Ubiquitin" mixture for 60 minutes at 30°C in a 20-µl reaction. After incubation, TR-FRET was detected (340=>620 nm, and 340=>665 nm). The TR-FRET is proportional to the auto polyubiquitination of TRAF6.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Ubiquitination