101597-1 101597-2
Scientist.com Supplier
TRAF6, GST-Tag Recombinant
BPS Bioscience
DESCRIPTION
Human Recombinant TRAF6 (TNF receptor associated factor 6), encompassing amino acids 51-159. This construct contains an N-terminal GST-tag followed by a PreScission Site to facilitate removal of the GST-tag.
DETAILS
- Aa: 51-159
- Mw: 39 kDa
- Tags: N-terminal GST-Tag
- Format: Aqueous buffer solution.
- Purity: ≥90%
- Genbank: NM_145803
- Species: Human
- Uniprot: Q9Y4K3
- Shiptemp: -80°C (dry ice)
- Synonyms: TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6, RNF85
- Category: Ubiquitination/Protein
- Constructs: TRAF6 (GST-Pre-51-159)
- Description: Human Recombinant TRAF6 (TNF receptor associated factor 6), encompassing amino acids 51-159. This construct contains an N-terminal GST-tag followed by a PreScission Site to facilitate removal of the GST-tag.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and variable Glutathione
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: E. coli
- Product Type: Protein
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: TRAF6 activity was measured using varying amounts of TRAF6 and incubated with E1, E2, and ATP in the presence of an "intrachain Ubiquitin" mixture for 60 minutes at 30°C in a 20-µl reaction. After incubation, TR-FRET was detected (340=>620 nm, and 340=>665 nm). The TR-FRET is proportional to the auto polyubiquitination of TRAF6.
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Ubiquitination