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SPN-B5283-50ug SPN-B5283-500ug

Bat coronavirus Spike Trimer Protein (K978P, V979P), His Tag&Twin-Strep Tag (MALS verified)

ACROBiosystems

DESCRIPTION

Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (SPN-B5283) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Gln 1200 (Accession # UAY13253.1 (K978P, V979P)).

DETAILS

  • Tag: C-10×His & C-Twin-Strep
  • Host: HEK293
  • Size: 50ug
  • Buffer: PBS
  • Format: Powder
  • Purity: 95%
  • Storage: -20℃
  • Category: Viral Proteins
  • Molecule: Spike protein
  • Accession: UAY13253.1 (K978P, V979P)
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Exp Region: Met 1 - Gln 1200
  • Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by a twin strep tag. The protein has a calculated MW of 141.2 kDa. The protein migrates as 170-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 141.2 kDa
  • Shipping Condition: RT
  • Species Reactivity: Bat coronavirus
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html