DESCRIPTION

• Mw: 1180.1 Da
• Format: Aqueous buffer solution
• Purity: ≥95% by HPLC
• Shiptemp: -80°C (dry ice)
• Synonyms: 6-Biotin-17-NAD+, Biotin Nicotinamide adenine dinucleotide, β- Nicotinamide-N6-(2-(6-[biotinyl] aminohexanoyl) aminoethyl) adenine dinucleotide, NAD
• Warnings: Avoid freeze/thaw cycles.
• Category: PARP/Substrates
• Background: Activated PARP cleaves NAD+ to nicotinamide and ADP-ribose and polymerizes the ADP-ribose on nuclear acceptor proteins such as histones, transcription factors, and PARP itself. Biotinylated NAD+ (6-Biotin-17-NAD+) provides a non-isotopic alternative to radiolabeled NAD+ for assays requiring this substrate. Biotinylated NAD+ can be used as an indirect measure of PARP activity when biotin incorporation is detected using a streptavidin detection system. Biotinylated NAD+ is also useful for labeling or purifying proteins via poly (ADP)ribosylation.
• References: 1. Bakondi, E., et al. J Histochem Cytochem January 2002 vol. 50 no. 1 91-98. 2. Zhang, J. (1997). Meth. Enzymol. 280: 255-65. 3. Till, S., and Ladurner, A.G. (2009). Front Biosci. 14:3246-3258. 4. Ying, W. (2007). Front Biosci. 12:1863-88.5. Ying, W. (2008). Antioxidants & Redox Signaling. 10(2):179-206. Application Reference: Activity-based assay for human mono-ADP-ribosyltransferases ARTD7/PARP15 and ARTD10/PARP10 aimed at screening and profiling inhibitors (2013)
• Description: NAD+ covalently labeled with biotin (6-biotin-17-nicotinamide-adenine-dinucleotide). Can be used as a substrate by poly(ADP-ribosyl) polymerase (PARP) enzymes for generating biotin-labeled ADP-ribose monomers in PARylation reactions.
• Supplied As: 250 µM aqueous solution
• Unspsc Code: 12352200
• Unspsc Name: Biochemicals
• Applications: Useful for the study of PARP enzyme activity and DNA damage, or to purify ADP-ribosylated enzymes.
• Cas Registry: 53-84-9 (NAD+)
• Product Type: Substrate
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 79572, 79512
• Storage Stability: Store solutions in aliquots at -80°C for up to one year.
• Scientific Category: PARP