40634-V08B
SARS-CoV Spike S1+S2 ECD-His Recombinant Protein (S577A, Isolate Tor2)
Sino Biological
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DESCRIPTION
A DNA sequence encoding the full-lenght ECD of SARS-CoV (isolate: Tor2) spike (NP_828851.1) (Met1-Pro1195) was expressed with a C-terminal polyhistidine tag.
DETAILS
- Tag: C-His
- Purity: > 90 % as determined by SDS-PAGE.
- Source: Baculovirus-Insect Cells
- Species: SARS
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by its binding ability in a functional ELISA. Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2μg/mL (100μL/well) can bind SARS-CoV Spike S1+S2 ECD-His Recombinant Protein (S577A, Isolate Tor2)(40634-V08B),the EC50 of SARS-CoV Spike S1+S2 ECD-His Recombinant Protein (S577A, Isolate Tor2)(40634-V08B) is 300-900ng/mL.
- Molecule: Spike
- Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile 20mM PB, 300mM NaCl, pH 7.0, 10% glycerol
- Molecular Mass: The recombinant SARS-CoV (isolate: Tor2) spike S1+S2 ECD consists of 1193 amino acids and predicts a molecular mass of 132.58 kDa.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_828851.1
- Predicted N Terminal: Ser14