302105-25
Calcium Free Apoptotic-dead Cell Tag -594
BioMagis
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DESCRIPTION
Calcium Free Apoptotic-Dead Cell Tag – 594 is a probe specifically designed to bind to phosphatidylserine (PS) exposed on the outer leaflet of the cell membrane in apoptotic cells and dead cells, regardless of calcium levels. This probe is suitable for detecting apoptotic cells, dead cells, activated platelets, and extracellular vesicles using flow cytometry or fluorescence microscopy. Moreover, it can be used in multicolor staining alongside conjugated antibodies or dead cell indicators in calcium-free buffers. Importantly, the staining performed with the calcium-free apoptotic-dead cell tag is fixable, enabling the distinction of viable cells for subsequent intracellular staining.
DETAILS
- Application: ICC
- Fluorophore: A594
- Formulation: Lyophilized
- Application Notes: Protocol for ICC 1. Gently remove the buffer from the adherent cells 2. Gently add blocking buffer (PBS plus 5% FCS or BSA) for 15 minutes at room temperature. 3. Decant the blocking buffer. 4. Take the appropriate volume of reconstitute Calcium free Apo-dead Cell Tag solution. Recommend add 5 µL of reagent in 100 µL of staining buffer to 1 million cells for 15 minutes at room temperature in dark. Notes: The optimal concentration of Calcium Free Apoptotic-Dead cell tag may be different for different type of cells. 5. Gently wash the cells once. 6. Mount the cells with antifade mounting medium for imaging. Note: Cells can be fixed after staining.
- Excitationemission: Ex 590 / Em 617 nm
- Verified Reactivity: Human, Mouse
- Storage and Handling: • Store at -20°C upon receipt. Do not open vials until needed • Once diluted to dilution buffer can be stored at 4-8°C for three months
- Reconstitution and Usage: Place the vial at room temperature for a couple of minutes follow with a quick spin. Reconstitute with 130 µL of dilution buffer. Recommend 5 µl per 100 µl of any staining buffer for 1 million cells