Monoclonal Antibody to Aldehyde dehydrogenase H1L1, ALDH1L1(Clone: 4A12)
Abeomics
Image
Figure-1: Immunofluorescent analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with mouse mAb to aldehyde dehydrogenase family 1 member L1 (ALDH1L1), (34-1004), dilution 1:1,000 in green, and costained with chicken pAb to vimentin,(34-1126), dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. (34-1004) antibody produces strong cytoplasmic staining of astrocytic glial cells, while the(34-1126) antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing asrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.
Image
Figure-2: Western blot analysis of rat and mouse tissue lysates using mouse mAb to ALDH1L1,(34-1004), dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.
DETAILS
Gene:
ALDH1L1
App Id:
WB, IF/ICC, IHC
Antigen:
Aldehyde dehydrogenase H1L1, ALDH1L1
Gene Id:
10840
Isotype:
Mouse, IgG1
Format Id:
Purified
Clone Name:
4A12
Uniprot Id:
O75891
Host Animal:
Mouse
Clonality Id:
Monoclonal
Reactivity Id:
Human, Rat, Mouse
Product Content:
Antibody is supplied as an aliquot of 1 mg/ml of affinity purified antibody or concentrated tissue culture supernatant.
Alternative Name:
Aldehyde dehydrogenase family 1 member L1