mouse monoclonal IgG1; vinculin Antibody (7F9) is an IgG1 κ mouse monoclonal vinculin antibody (also designated Metavinculin antibody or VCL antibody) that detects the vinculin protein of human, mouse, rat and avian origin by WB, IP, IF and IHC(P). vinculin Antibody (7F9) is available as both the non-conjugated anti-vinculin antibody form, as well as multiple conjugated forms of anti-vinculin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.