50118
JMJD2E (KDM4DL), GST-tag Recombinant
BPS Bioscience
DESCRIPTION
Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system.
DETAILS
- Aa: 2-337
- Mw: 65 kDa
- Tags: N-terminal GST-tag
- Format: Aqueous buffer solution
- Genbank: NM_001161630
- Species: Human
- Uniprot: B2RXH2
- Shiptemp: -80°C (dry ice)
- Synonyms: KDM4DL, JMJDE, KDM4D-Like
- Warnings: Avoid freeze/thaw cycles.
- Category: Demethylase/Enzymes
- References: 1. Fodor, B.D., et al., (2006). Genes Dev. 20(12): 1557-1562. 2. Beyer, S., et al., (2008). J. Biol. Chem. 283(52):36542-52. Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
- Description: Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2E (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
- Related Products: 50417, 50105, 50117, 50111
- Specific Activity: 0.14 pmole/min/µg
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Demethylase