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50118

JMJD2E (KDM4DL), GST-tag Recombinant

BPS Bioscience

DESCRIPTION

Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system.

DETAILS

  • Aa: 2-337
  • Mw: 65 kDa
  • Tags: N-terminal GST-tag
  • Format: Aqueous buffer solution
  • Genbank: NM_001161630
  • Species: Human
  • Uniprot: B2RXH2
  • Shiptemp: -80°C (dry ice)
  • Synonyms: KDM4DL, JMJDE, KDM4D-Like
  • Warnings: Avoid freeze/thaw cycles.
  • Category: Demethylase/Enzymes
  • References: 1. Fodor, B.D., et al., (2006). Genes Dev. 20(12): 1557-1562. 2. Beyer, S., et al., (2008). J. Biol. Chem. 283(52):36542-52. Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
  • Description: Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system.
  • Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
  • Unspsc Code: 12352202
  • Unspsc Name: Proteins
  • Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
  • Host Species: insect
  • Product Type: Protein
  • Host Cell Line: Sf9 cells
  • Biosafety Level: Not applicable (BSL-1)
  • Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2E (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
  • Related Products: 50417, 50105, 50117, 50111
  • Specific Activity: 0.14 pmole/min/µg
  • Storage Stability: At least 6 months at -80°C.
  • Scientific Category: Demethylase