DESCRIPTION

• Aa: 2-337
• Mw: 65 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_001161630
• Species: Human
• Uniprot: B2RXH2
• Shiptemp: -80°C (dry ice)
• Synonyms: KDM4DL, JMJDE, KDM4D-Like
• Warnings: Avoid freeze/thaw cycles.
• Category: Demethylase/Enzymes
• References: 1. Fodor, B.D., et al., (2006). Genes Dev. 20(12): 1557-1562. 2. Beyer, S., et al., (2008). J. Biol. Chem. 283(52):36542-52. Application Reference(s): A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
• Description: Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2E (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
• Related Products: 50417, 50105, 50117, 50111
• Specific Activity: 0.14 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Demethylase