Anti-Vav2 Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: VAV2
Host: Rabbit
Rrid: AB_1184726
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Vav2
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: VAV2, Guanine nucleotide exchange factor VAV2
Clonality: Rabbit Polyclonal
Immunogen: Vav2 synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-172 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 100 kDa* band corresponding to Vav2 on SDS-PAGE immunoblots of human A431 cells and detects several Vav isoforms in Jurkat cells.
Applications: WB, ICC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 100
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Mouse
Dilution Range Icc: 1:50
Species Reactivity: Human, Mouse, and Rat
Target Description: The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.
Uniprot Number Immunogen Species: Q60992