DESCRIPTION

• Aa: 121-end
• Mw: 66 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_002603
• Species: Human
• Uniprot: Q13946
• Shiptemp: -80°C (dry ice)
• Synonyms: PDE7A, phosphodiesterase 7A
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Enzymes
• References: 1. Wang, H., et al., J. Biol. Chem. 280(35):30949-30955 (2005).2. Torras-Llort, M. and Azorin, F., Biochem. J. 373(PT 3):835-843 (2003). Application References: 1. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014) 2. PDE7A1 hydrolyzes cCMP (2014) The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)
• Description: Human PDE7A1 GenBank Accession No. NM_002603), a.a. 121-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM Glutathione, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
• Assay Conditions: Assayed using BPS's PDE7A kit, Cat. # 60370. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE7A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
• Related Products: 60370, 60407, 60413, 60373
• Specific Activity: ≥ 180 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Phosphodiesterase