DESCRIPTION

• Aa: 359-635(end)
• Mw: 58 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_003177
• Species: Human
• Uniprot: P43405
• Shiptemp: -80°C (dry ice)
• Synonyms: SYK, Spleen Tyrosine Kinase
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Cytoplasmic Tyrosine
• References: 1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41.2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.
• Description: Recombinant human SYK, catalytic subunit (GenBank Accession # NM_003177), a.a. 359-635(end) with an N-terminal GST-tag, was expressed in Sf9 insect cells using a baculovirus expression system. MW = 58 kDa.
• Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: SYK was incubated with a substrate (Tyr peptide 2) for 1 hour at RT in 1X kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
• Related Products: 78487
• Specific Activity: 139 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase/Cytoplasmic Tyr