DESCRIPTION

• Notes: License Disclosure: Visit bpsbioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
• Species: SARS-CoV-2
• Shiptemp: -80°C (dry ice)
• Synonyms: coronavirus, covid, covid-19, covid19, Sars-cov-2 B.1.1.28.1 (P.1), sars cov 2, sarscov2, Brazil variant, Brazilian lenti, P1 lentivirus, pseudovirus, pseudovirion, 78143-1, 78143-2
• Warnings: Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
• Category: Coronavirus/Lentivirus
• Background: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of the viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein and ACE2 may offer protection against the viral infection. In Brazil, a variant called B.1.1.28.1 (P.1) was first identified in the fall of 2020. This variant has many mutations which may lead to higher transmissibility and infectivity. Among these mutations, three (K417T, E484K, N501Y) appear to be crucial.
• Constructs: K417T, E484K, N501Y
• Description: The Spike (K417T, E484K, N501Y) (SARS-CoV-2) Pseudotyped Lentiviruses were produced with SARS-CoV-2 Variant Spike (Genbank Accession #QHD43416.1 with mutations K417T, E484K, and N501Y) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions contain the firefly luciferase gene driven by a CMV promoter, therefore, the spike-mediated cell entry can be measured via luciferase activity. The Spike (SARS-CoV-2, K417T, E484K, N501Y) pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 K417T, E484K, N501Y variant in intact cells using a Biosafety Level 2 facility.
• Formulation: The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.
• Supplied As: The titer will vary with each lot; the exact value is provided with each shipment.
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: 1. Study the mechanism of viral transduction of SARS-CoV-2 K417T, E484K, N501Y variant.2. Screening for neutralizing antibodies that inhibit the binding of SARS-CoV-2 Spike (K417T, E484K, N501Y variant) to ACE2.
• Product Type: Lentivirus
• Biosafety Level: BSL-2
• Related Products: 60690, 60187, 79942, 78112
• Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
• Scientific Category: Coronavirus
• Instructions for Use: See assay protocol for detailed instructions.