CFS-EDX-DB
Disulfide Bond PLUS Expression Kit
CellFree Sciences
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DESCRIPTION
Modified version of the PLUS Expression Kit coming with DTT-free translation buffer and added PDI/Ero1α enzyme mix to support formation of disulfide bonds. Transcription and translation reagents are premixed for easy handling. Kit uses bilayer reaction on 226 μl scale. This kit contains an expression vector, a positive control, and a PCR primer set.
DETAILS
- Features: Modified reaction conditions to support formation of disulfide bonds in cell-free expression reactions Kit provides Easy setup of cell-free protein expression experiments using premixed reagents Protein expression reactions can be maintained for up to 24 hours using bilayer format Kit provides expression vector and PCR primers for template preparation tPA positive control vector included Manual provided as download file
- Tag Line: Preparing proteins having disulfide bonds
- Applications: Preparation of proteins having disulfide bonds Testing protein expression under modified reaction conditions Disulfide bonds can increase protein solubility and activity
- Storage Condition: -80 ℃