mouse monoclonal IgG1; Calnexin Antibody (TO-5) is an IgG1 κ mouse monoclonal Calnexin antibody (also designated Calnexin antibody or CANX antibody) that detects Calnexin of human origin by WB, IP, IF, IHC(P) and FCM. Calnexin Antibody (TO-5) is available as the non-conjugated anti-Calnexin antibody form, as well as multiple conjugated forms of anti-Calnexin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Calnexin and Calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.