DESCRIPTION

• Aa: 1-749 (end)
• Mw: 87 kDa
• Tags: C-terminal FLAG-His-tags
• Format: Aqueous buffer solution
• Genbank: NM_024420
• Species: Human
• Uniprot: P47712
• Shiptemp: -80°C (dry ice)
• Synonyms: Phospholipase A2 G4
• Warnings: Avoid freeze/thaw cycles.
• Category: Phospholipase
• References: 1. Chalimoniuk M. Postepy Biochem. 2012; 58(2):204-8.2. Nadalin S, Giacometti J, et al., Prostaglandins Leukot Essent Fatty Acids. 2013; 88(2):185-90.
• Description: Human PLA2G4A, GenBank Accession No. NM_024420, a.a. 1-749 (end), with C-terminal FLAG-His-tag, MW=87 kDa, expressed in a baculovirus expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, and 20% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: PLA reaction was performed using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine substrate. Enzyme reaction is conducted in 100 µl volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl2, 0.5 mg/ml BSA, 10 µM substrate and 200 ng PLA2G4A. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature.
• Specific Activity: ≥57 pmol/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Phospholipases