Anti-N-WASP (Ser-484/Ser-485), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: WASL
Host: Rabbit
Rrid: AB_2217122
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: N-WASP (Ser-484/Ser-485)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Neural Wiskott-Aldrich syndrome protein, WASL, WASP
Clonality: Rabbit Polyclonal
Immunogen: Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide then affinity purified using phospho-N-WASP (S484/S485) peptide. The antibody detects a 63 kDa* protein corresponding to the molecular mass of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa* protein corresponding to phosphorylated N-WASP.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 63
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:50
Species Reactivity: Human, Mouse, and Rat
Target Description: Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types
Uniprot Number Immunogen Species: O00401
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-484/Ser-485