DESCRIPTION

• Aa: full length
• Mw: 135 kDa (complex)
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_001258 (CDK3), NM_001238 (Cyclin E)
• Species: Human
• Uniprot: Q00526 (CDK3), P24864 (Cyclin E)
• Shiptemp: -80°C (dry ice)
• Synonyms: Cyclin-Dependent Kinase 3, Cell Devision Kinase 3, CDK3, Cyclin E1, CCN1, CCNE1
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• References: 1. Bullrich, F. et al., Cancer Res. (1995); 55:1199-1205. 2. Ye, X. et al., Proc. Nat. Acad. Sci. (2001); 98:1682-1686.
• Description: Complex of N-terminal GST-tagged recombinant full length human CDK3 (GenBank Accession No. NM_001258), and Cyclin E1 (GenBank Accession No. NM_001238). Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK3 MW=61 kDa, Cyclin E1 MW=74 kDa.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for three dimensional structure studies, binding assays, enzyme kinetics, screening inhibitors, and selectivity profiling.
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 µM ATP at 30°C for 50 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
• Related Products: 40104, 40102, 40454, 40206, 78487
• Specific Activity: 30 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine