21005C-1mL 21005C-10mL 21005C-100mL
MagVigen™ - Streptavidin, cell seperation
NVIGEN
DESCRIPTION
Product Description
MagVigen™-Streptavidin magnetic nanoparticles can universally bind to any biotinylated biomolecules (ex. antibody, protein, peptide, DNA) through high affinity interaction between streptavidin and biotin. The MagVigen™-Streptavidin-biotin-biomolecule complex can be easily separated from unbound biotin-biomolecule using a magnetic rack (Cat#A20006). This provides a quick and neat way to tag biomolecules with magnetic nanoparticles. The purified nanoparticle-biomolecule complex can be used in a variety of downstream bio-separation processes (ex. protein purification, immunoprecipitation, cell isolation or depletion, and molecular detection.)
MagVigen™-Streptavidin are ideally used together with mouse antibody for isolation of cells (e.g. CTCs, stem cells) from a mixture of cell population obtained from tissues or organs. The isolated cells are viable and can be further cultured or used for downstream molecular analysis such as mRNA isolation and RT-PCR. Cell separation with MagVigen™ nanoparticles eliminates the use of columns, so cells are not exposed to the mechanical stress from passing through the column matrix. Magnetically separated cells are highly purified and retain their viability, ideal for downstream applications.
Advantages of MagVigen™ – Streptavidin for cell enrichment
Easy and quick to make nanoparticle-primary mouse antibody conjugates
Simple and gentle cell separation
Strong and long-lasting fluorescent signal
Consistent, high quality results
High binding capacity
High biocompatibility
Low non-specific binding
DETAILS
- Category: Purification Reagent
- Lead Time: 1 week
- Subcategory: Magnetic Beads
- Applications: Cell purficiation
- Product and Related Product Contents: Cat# 21005C: MagVigen™- Streptavidin are provided in phosphate buffered saline (PBS) containing 0.02% NaN3, 0.02% BSA. pH 7.4. Each vial contains 1 ml of solution with particle concentration of 2 mg/ml, which is enough for binding 109 cells. Nanoparticle size: 200-500 nm measured using Dynamic Light Scattering. Polydispersity index < 0.2. Capacity: 50μg biotin-antibody/ml of nanoparticles All materials except the magnet should be stored at 4°C. Shelf life: 6 months