DESCRIPTION

• Form: Lyophilized powder
• Host: Rabbit
• Clone: NA
• Label: ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
• Purity: Affinity purified on immobilized antigen.
• Target: Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 24418
• Isotype: Rabbit IgG
• Homology: Mouse - identical; human - 13/14 amino residues identical
• Is Toxin: No
• Sequence: EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5
• Synonyms: Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
• Accession: P31424
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Rat|Mouse
• Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
• Applications: IF|IHC
• Preservative: 1% BSA, 0.05% NaN3
• Reconstitution: 50 µl double distilled water (DDW).
• Blocking Peptide: BLP-GC007
• Negative Control: BLP-GC007
• Positive Control: NA
• Immunogen Location: Extracellular, N-terminus
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Rat
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: IF|IHC
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus.The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.
• Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #AGC-007), and immunohistochemistry (labeled antibody).
• Application Dilutions Western Blot Wb: 1:50-1:60
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information