PD1-H52H2-100ug PD1-H52H2-500ug PD1-H52H2-1mg
Human PD-1 Protein, His Tag (Nanoparticle)
ACROBiosystems
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DESCRIPTION
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
DETAILS
- Tag: C-10×His
- Host: HEK293
- Size: 1mg
- Buffer: PBS with glycine and sodium citrate, pH8.0
- Format: Powder
- Purity: 90%
- Source: Human PD-1 Protein, His Tag (PD1-H52H2) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
- Storage: -20℃
- Category: Immune Checkpoint Proteins
- Molecule: PD-1
- Accession: NP_005009.2
- Conjugate: Unconjugated
- Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Exp Region: Leu 25 - Gln 167
- Swiss-prot: Q15116-1
- Package Size: 1mg*1
- Characteristics: Human PD-1 Protein, His Tag is designed and expressed as nanoparticles of approximately 25 nm diameter by displaying PD-1 ECD in native conformation. The nanoparticles each was coated with an average of 180 PD-1 ECD. The nanoparticle technology allows directional and high-density display of human PD-1 on the surface of nanoparticles, thus greatly enhances the immune response in vivo to otherwise poorly immunogenic epitopes. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.1 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Endotoxin Level: 0.5 EU per μg
- Molecular Weight: 47.1 kDa
- Shipping Condition: RT
- Species Reactivity: Human
- Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html