Recombinant human Irisin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-28 kDa..
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The purity of Human/Mouse/Rat Irisin Protein (Cat.RP01164) was greater than 90% as determined by SEC-HPLC.
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3T3-L1 preadipocytes were incubated with 1 pg/mL-0.1μg/mL of Recombinant Human Irisin for 12 minutes. The cells were lysed and Western blots were performed with anti-phospho p38 MAPK and total p38 MAPK antibodies.