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40595-V08B

SARS-CoV-2 (2019-nCoV) RNA-dependent RNA polymerase / RDRP Protein (His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) RdRp (YP_009725307.1) (Ser1-Gln932) was expressed with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.

DETAILS

  • Tag: C-His
  • Purity: > 85 % as determined by SDS-PAGE.
  • Source: Baculovirus-Insect Cells
  • Species: SARS-CoV-2
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Molecule: RDRP-CoV
  • Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol.
  • Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) RdRp consists of 944 amino acids and predicts a molecular mass of 108.3 kDa. It migrates as an approximately 91.3 kDa band in SDS-PAGE under reducing conditions.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: YP_009725307.1
  • Predicted N Terminal: Met