DESCRIPTION

• Note: Single siRNA duplex (10nmol) can be ordered.
• Purity: HPLC purified
• Refseq: NM_027927
• Status: 3 Weeks*
• Summary: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.[UniProtKB/Swiss-Prot Function]
• Category: RNAi
• Synonyms: 1110020M19Rik; 2810027J24Rik; 4930529N21Rik; A230056J18Rik; AI666778; int12; Phf22
• Sequences: Available with shipment
• Stability: One year from date of shipment when stored at -20°C.
• Components: Ints12 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 71793)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml Need an individual siRNA?
• Uniprot Id: Q9D168
• Availability: 3 Weeks
• Quality Control: Tested by ESI-MS
• Performance Guaranteed: OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
• Number of Transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).