DESCRIPTION

• Aa: 632-1304
• Mw: 81 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_002838
• Species: Human
• Uniprot: P08575
• Shiptemp: -80°C (dry ice)
• Synonyms: CD45, LCA, T200, LY5, B220, GP180
• Warnings: Avoid freeze/thaw cycles.
• Category: Protein Phosphatase/Receptor
• Background: PTPRC (CD45) contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.
• References: 1. Rachmilewitz, J. et al., J. Biol. Chem. 278, 14059-14065. 2. Fernandis, A.Z. et al., J. Biol. Chem. 278, 9536-9543.
• Description: Protein tyrosine phosphatase CD45, also known as PTPRC (GenBank accession No. NM_080922), catalytic domain, a.a. 632-1304 (end) with N-terminal His-tag, MW=81 kDa, expressed in a Baculovirus Sf9 expression system.
• Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM PNPP, 20 min at room temperature. Absorbance was measured at 405 nm. Specific activity was calculated based on _x0001_pnp_x0002_=18,000M-1cm-1.
• Specific Activity: 341 pmole/min/µg
• Storage Stability: >12 months at -80°C
• Scientific Category: PTP/Receptor