Anti-Crk II (Tyr-221), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CRK
Host: Rabbit
Rrid: AB_3068411
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Crk II (Tyr-221)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: cCrk, c-CRK
Clonality: Rabbit Polyclonal
Immunogen: Phospho-Crk (Tyr-221) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-221 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, and has 40% homology with the conserved site in CrkL (Tyr-207). The site is not found in Crk I.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk II on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 40
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Chicken, Human, Mouse, Rat, and Xenopus
Target Description: The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl.
Uniprot Number Immunogen Species: P46108
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Tyr-221