ELISA for Quantitative Determination of Serratia endonuclease in Cell Culture and Biological Samples Serratia marcescens nuclease originates from Gram negative bacteria S. marcescens and heads a family of homological non-specific nucleases that are widely spread in the world. Serratia nuclease is most studied one and it is capable to cleavage both RNA and DNA in either single or double stranded form. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The Endonuclease Serratia marcescens ELISA is a competitive immunoassay for the determination of Serratia endonuclease in sample. The secondary antibodies specific to anti-endonuclease are coated on to the microtiter plate. A constant concentration of HRP labeled endonuclease and varying concentration of standard or sample containing endonuclease compete for binding to anti-endonuclease antibodies. This immune complex is captured by the coated secondary antibody. After incubation and washing, the unbound labeled enzyme is removed. Then addition of substrate develops blue color during incubation period and the reaction is stopped after the addition of stop solution with development of yellow color. The intensity of the color generated is inversely proportional to the amount of endonuclease in the sample.