Anti-CD300a Monoclonal Antibody (Clone:MEM-260)

DESCRIPTION

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.

DETAILS

Gene: CD300A
App Id: FACS, WB
Antigen: CD300a
Gene Id: 11314
Isotype: Mouse IgG1
Format Id: Purified
Clone Name: MEM-260
Uniprot Id: Q9UGN4
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Purified by protein-A affinity chromatography
Reactivity Id: Human
Alternative Name: CD300A||CMRF35H||IGSF12||HSPC083
Application Note: Flow Cytometry Recommended dilution:2 µg/ml Western Blotting Positive material: HPB cell line Negative material: JURKAT human cell line, RAJI human cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Storage Condition: Store at 2-8°C. Do not freeze.
Immunogen Information: HPB human acute lymphoid leukemia cell line