Anti-Cofilin 1 (N-terminus) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CFL1
Host: Rabbit
Rrid: AB_2260620
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Cofilin 1 (N-terminus)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: ADF, p18
Clonality: Rabbit Polyclonal
Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the N-terminus of human Cofilin 1. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has 3 amino acid differences from human Cofilin 2.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 19 kDa* protein corresponding to the molecular mass of Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 19
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:50
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.
Uniprot Number Immunogen Species: P23528