DESCRIPTION

• Mw: 35.3 kDa
• Tag: Myc-DDK
• Accn: NM_001126122
• Type: Human Tagged ORF Clone
• Refseq: NM_001126122.1, NP_001119594.1
• Status: 3 Weeks*
• Symbol: SLC25A19
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 60386
• Orf Size: 960 bp
• Synonyms: DNC; MCPHA; MUP1; THMD3; THMD4; TPC
• Refseq Orf: 963 bp
• Uniprot Id: Q9HC21
• Availability: 3 Weeks
• Cytogenetics: 17q25.1
• Gene Summary: This gene encodes a mitochondrial protein that is a member of the solute carrier family. Although this protein was initially thought to be the mitochondrial deoxynucleotide carrier involved in the uptake of deoxynucleotides into the matrix of the mitochondria, further studies have demonstrated that this protein instead functions as the mitochondrial thiamine pyrophosphate carrier, which transports thiamine pyrophosphates into mitochondria. Mutations in this gene cause microcephaly, Amish type, a metabolic disease that results in severe congenital microcephaly, severe 2-ketoglutaric aciduria, and death within the first year. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC225460 representing NM_001126122 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGTTGGCTATGACCCCAAACCAGATGGCAGGAATAACACCAAGTTCCAGGTGGCAGTGGCTGGGTCTG TGTCTGGACTTGTTACTCGGGCGCTGATCAGTCCCTTCGACGTCATCAAGATCCGTTTCCAGCTTCAGCA TGAGCGCCTGTCTCGCAGTGACCCCAGCGCAAAGTACCATGGCATCCTCCAGGCCTCTAGGCAGATTCTG CAGGAGGAGGGTCCGACAGCTTTCTGGAAAGGACACGTCCCAGCTCAGATTCTCTCCATAGGCTATGGAG CTGTCCAATTCTTGTCATTTGAAATGCTGACGGAGCTGGTCCACAGAGGCAGCGTGTACGACGCCCGGGA ATTCTCAGTGCACTTTGTATGTGGTGGCCTGGCTGCCTGTATGGCCACCCTCACTGTGCACCCCGTGGAT GTTCTGCGCACCCGCTTTGCAGCTCAGGGTGAGCCCAAGGTCTATAATACGCTGCGCCACGCCGTGGGGA CCATGTATAGGAGCGAAGGCCCCCAGGTTTTCTACAAAGGCTTGGCTCCCACCTTGATCGCCATCTTCCC CTACGCCGGGCTGCAGTTCTCTTGCTACAGCTCCTTGAAGCACCTGTACAAGTGGGCCATACCAGCCGAA GGAAAGAAAAATGAGAACCTCCAAAACCTGCTTTGTGGCAGTGGAGCTGGTGTCATCAGCAAGACCCTGA CATATCCGCTGGACCTCTTCAAGAAGCGGCTACAGGTTGGAGGGTTTGAGCATGCCAGAGCTGCCTTTGG CCAGGTACGGAGATACAAGGGCCTCATGGACTGTGCCAAGCAGGTGCTACAAAAGGAAGGCGCCCTGGGC TTCTTCAAGGGCCTGTCCCCCAGCTTGCTGAAGGCTGCCCTCTCCACAGGCTTCATGTTCTTCTCGTATG AATTCTTCTGTAATGTCTTCCACTGCATGAACAGGACAGCCAGCCAGCGC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC225460 representing NM_001126122 Red =Cloning site Green =Tags(s) MVGYDPKPDGRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQIL QEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVD VLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHLYKWAIPAE GKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALG FFKGLSPSLLKAALSTGFMFFSYEFFCNVFHCMNRTASQR TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Druggable Genome
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin