DESCRIPTION

• Aa: 447-761
• Mw: 39 kDa
• Tags: N-terminal His-Avi-tag
• Format: Aqueous buffer solution
• Genbank: NM_00142
• Species: Human
• Uniprot: P22607
• Shiptemp: -80°C (dry ice)
• Synonyms: JTK4, CD333, CEK2, ACH
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Protein
• References: 1. Cappellen, D. et al., Nature Genetics. (1999); 23:18-20.2. Bellus, G.A. et al., AM. J. Hum. Genet. (1995); 56(2):368-373.
• Description: Human Fibroblast growth factor receptor 3 (FGFR3), also known as JTK4, CD333, CEK2 and ACH, GenBank Accession No. NM_00142, a.a. 447-761, with N-terminal His-Avi-tag, expressed in an Sf9 infected baculovirus expression system and enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be ≥90%. MW = 39 kDa. This protein was isolated from a co-expression with PTP1B. Percentage of phosphorylation ≤5%.
• Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
• Supplied As: Aqueous buffer solution.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assay was done in Kinase buffer containing 2 mM DTT using 0.2 mg/mL Poly-(Glu:Tyr) substrate and 10 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
• Related Products: 40212, 100140, 100138, 100142, 78487
• Specific Activity: 4.3 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase