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ANR-082-AR_50 mcl

Scientist.com Supplier

Anti-N-Cadherin-ATTO Fluor-594 Antibody

Alomone Labs

DESCRIPTION

Anti-N-Cadherin Antibody (#ANR-082) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize CDH2 from rat, mouse, and human samples. Anti-N-Cadherin-ATTO Fluor-594 Antibody (#ANR-082-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-N-Cadherin-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

DETAILS

  • Form: Lyophilized powder
  • Host: Rabbit
  • Clone: NA
  • Label: ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
  • Purity: Affinity purified on immobilized antigen.
  • Target: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal
  • Comment: Contact Alomone Labs for technical support and product customization
  • Gene Id: 12558
  • Isotype: Rabbit IgG
  • Homology: Rat, human - identical
  • Is Toxin: No
  • Sequence: (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2
  • Synonyms: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal
  • Accession: P15116
  • Clonality: Polyclonal
  • Lead Time: 1-2 Business Days
  • Reactivity: Human|Rat|Mouse
  • Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
  • Applications: IF|IHC
  • Preservative: 1% BSA, 0.05% NaN3
  • Reconstitution: 50 µl double distilled water (DDW).
  • Blocking Peptide: BLP-NR082
  • Negative Control: BLP-NR082
  • Positive Control: NA
  • Immunogen Location: Intracellular
  • Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
  • Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
  • Immunogen Source Species: Mouse
  • Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
  • Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
  • Applications May Also Work In: IF|IHC
  • Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
  • Product Page - Scientific Background: Cadherins are transmembrane proteins responsible for cell adhesion. Cell adhesion is a crucial feature for the sustainability and maintenance of normal tissue function and three dimensional structure. Cadherins share an extracellular domain consisting of multiple repeats of cadherin-specific motif and are calcium dependent homotypic cell-to-cell adhesion molecules. They are localized mostly in specialized sites called adherence junctions1.N-cadherin consists of an amino-terminal external domain with five tandem repeats, a single transmembrane segment, and a cytoplasmic carboxy-terminal domain of approximately 150 amino acids. The intracellular domain of N-cadherin interacts with a group of proteins called catenins that are essential for cadherin-mediated cell adhesion. It is suggested that N-cadherin proteins align in a form of "zipper" when involved in cell adhesion. Cadherins on one cell surface form a series of rigid dimers that attach to equivalent dimers on the opposing cells and lateral motion of these complexes allows the cell junction site to "zip up"1.N-cadherin plays a role in tumor metastasis. Unlike E-cadherin that causes tumor metastasis when insufficiently, N-cadherin is involved in tumor metastasis when upregulated. N-cadherin stimulates migration, invasion and metastasis of squamous cell breast cancer and its effects are increased by FGF-2 suggesting that N-cadherin and FGFR-1 synergize and promote aggressive tumor behavior. This synergy also promotes neuronal growth1. Interestingly, low levels of N-cadherin are associated with a higher invasive capacity of astrocytic glioma by increasing tumor (and normal) cell migration speed and creating a less organized pattern of migration3.
  • Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #ANR-082), and Immunohistochemical staining (labeled antibody).
  • Application Dilutions Western Blot Wb: Contact Alomone for information
  • Antibody Concentration After Reconstitution: 1 mg/ml
  • Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:200