Monoclonal Antibody to EGFR (Epidermal Growth Factor Receptor)(Clone : 31G7)

DESCRIPTION

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

DETAILS

Gene: EGFR
App Id: FACS,IF,IHC
Antigen: EGFR (Epidermal Growth Factor Receptor)
Gene Id: 1956
Isotype: Mouse IgG2a, kappa
Format Id: Purified
Clone Name: 31G7
Uniprot Id: P00533
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: EGFR||ERBB||ERBB1||HER1
Application Note: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Digest formalin-fixed tissues with Protease (Sigma Cat. #P-6911) at 1mg/ml PBS, pH 7.4 for 10 min at 37C)
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Human EGFR purified from A431 cells