PCSK9 (D374Y) His-Avi-Tag, Biotin-Labeled Recombinant

DESCRIPTION

Recombinant human PCSK9 (Human proprotein convertase subtilisin/kexin 9) encompassing amino acids 31-692, which comprise the protodomain, the catalytic, and the C-terminal domains. The construct contains a C-terminal His-tag (6xHis) followed by an Avi-tag™. The protein contains the potent gain of function D374Y mutation. The protein was affinity purified.

DETAILS

Aa: 31-692(end)
Mw: Pro: 14 kDa; Mature: 60 kDa + glycans
Tags: C-terminal Avi-Tag™, His-Tag
Format: Aqueous buffer solution.
Purity: ≥90%
Genbank: NM_174936
Species: Human
Uniprot: Q8NBP7
Mutation: D374Y
Shiptemp: -80°C (dry ice)
Synonyms: Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1 (NARC-1), Proprotein convertase 9 (PC9), Subtilisin/kexin-like protease PC9, NARC1, PSEC0052
Warnings: Avoid freeze/thaw cycles
Category: Cholesterol/Protein
Background: PCSK9 (Proprotein convertase subtilisin/kexin type 9) functions as a negative regulator of hepatic low-density lipoprotein receptors (LDLRs) by binding to the LDLR ectodomain. The D374Y mutation is a gain of function mutation. The D374Y mutation is associated with hypercholesterolemia. This PCSK9 mutant is more potent at decreasing LDL uptake than wild-type PCSK9, most likely by increasing the binding affinity of PCSK9 for the LDLR. A deeper understanding of the function of this mutant protein and development of specific inhibitors may result in new therapeutic approaches for PCSK9 D374Y-linked diseases.
Constructs: PCSK9 (D374Y) (31-692(end)-His-Avi)(Biotin)
Description: Recombinant human PCSK9 (Human proprotein convertase subtilisin/kexin 9) encompassing amino acids 31-692, which comprise the protodomain, the catalytic, and the C-terminal domains. The construct contains a C-terminal His-tag (6xHis) followed by an Avi-tag™. The protein contains the potent gain of function D374Y mutation. The protein was affinity purified.
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: SDS-PAGE and pull-down assays.
Host Species: HEK293
Product Type: Protein
Biosafety Level: Not applicable (BSL-1)
Related Products: 72002, 71207, 71206
Storage Stability: At least 6 months at -80°C.
Scientific Category: Cholesterol-Related
Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Signed License Required: No