DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: CASP3, caspase 3, CPP32, apopain, Yama
• Warnings: Avoid freeze/thaw cycles.
• Category: Apoptosis/Assay Kit
• Background: Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.
• References: 1. Liu, J., et al., Bioorg. Med. Chem. Lett., 1999 Nov 15;9(22):3231-6.2. Riedl, S.J., et al., Cell, 2001 Mar 9;104(5):791-800
• Description: The Homogeneous Caspase-3 Assay Kit is a complete assay system designed to measure Caspase-3 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-3 activity measurements. In addition, the kit includes purified Caspase-3 enzyme and a potent Caspase-3 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-3 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-3 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.
• Methodology: Fluorogenic
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Protease
• Instructions for Use: See assay kit datasheet for detailed protocol.