40589-V49B1-B
SARS-CoV-2 Spike S1+S2 (LAL242-244 deletion, D80A, R246I, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His & AVI Tag), Biotinylated
Sino Biological
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DESCRIPTION
A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations LAL242-244 deletion, D80A, R246I, K417N, E484K, N501Y, D614G, A701V) (Met1-Pro1213) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as B.1.351) which emerged in the South Africa.
DETAILS
- Tag: C-His & AVI
- Purity: > 90 % as determined by SDS-PAGE.
- Source: Baculovirus-Insect Cells
- Species: SARS-CoV-2
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Molecule: Spike
- Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0.
- Molecular Mass: The recombinant SARS-CoV-2 Spike S1+S2 consists of 1221 amino acids and predicts a molecular mass of 135.82 kDa.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: YP_009724390.1
- Predicted N Terminal: Val 16