IL-8 Promoter Luciferase Reporter Lentivirus

DESCRIPTION

The IL-8 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-8 promoter. After transduction, activation of the IL-8 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

DETAILS

Notes: Biosafety The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Shiptemp: -80°C (dry ice)
Synonyms: IL-8 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter
Warnings: Avoid freeze/thaw cycles.
Category: Cell Line/Lentiviruses
Description: The IL-8 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-8 promoter. After transduction, activation of the IL-8 signaling pathway in the target cells can be monitored by measuring the luciferase activity.
Formulation: The lentiviruses were produced from HEK293T cells and are provided in medium containing 90% DMEM + 10% FBS.
Supplied As: Two vials (500 µl x 2) of IL-8 promoter luciferase reporter lentivirus at a titer >5 x 10^6 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Applications: • Screen for activators or inhibitors of IL-8 signaling pathway in transduced target cells • Generation of IL-8 promoter luciferase reporter stable cell line
Product Type: Lentivirus
Biosafety Level: BSL-2
Related Products: 71274-1, 60690, 60184, 79578, 79564
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Scientific Category: Cell Signaling Pathway
Instructions for Use: See assay protocol for detailed instructions.