40069
LKB1/MO25/STRAD Complex Recombinant
BPS Bioscience
DETAILS
- Aa: LKB1 2-433, MO25 2-341, STRAD 2-431
- Mw: 189 kDa (complex)
- Tags: STK11, N-terminal His-tag, MO25, N-terminal GST-tag, STRAD, N-terminal GST-tag
- Format: Aqueous buffer solution
- Genbank: STK11 (NM_000455), MO25 (NM_016289), STRAD (NM_001003787)
- Species: Human
- Uniprot: LKB1: Q15831; MO25: Q9Y376; STRAD: Q7RTN6
- Shiptemp: -80°C (dry ice)
- Synonyms: LKB1, STK11, PJS, Liver Kinase B1, STRAD, STE20-related kinase adaptor, LYK5, PMSE, MO25, Calcium Binding Protein 39
- Warnings: Avoid freeze/thaw cycles.
- Category: Kinase-Serine/Threonine/Protein
- References: 1. Yoo, L.I., et al. (2002). Nat. Rev. Cancer 2(7): 529-535. 2. Katajisto P, et al. (2007). Biochim. Biophys. Acta 1775 (1): 63-75.
- Description: Complex of human LKB1, also known as STK11 (GenBank Accession No. NM_000455, a.a. 2-433, with N-terminal His-tag), MO25 (GenBank Accession No. NM_016289, a.a. 2-341, with N-terminal GST-tag), and STRAD (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag). Calculated MW=49 kDa* (LKB1), 66 kDa (MO25), and 74 kDa (STRAD), expressed in Sf9 cells using a Baculovirus expression system. *LKB1 runs anomalously on SDS-PAGE.
- Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: 100 µM ATP and 0.2 mg/ml LKBtide peptide was used as a substrate. Reaction was incubated at 30°C for 30 min. Reaction progress was monitored using ADP-Glo reagents (Promega).
- Related Products: 40274, 40149, 101475, 101476, 78487
- Specific Activity: 24 pmol/min/µg
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Kinase-Serine/Threonine
DESCRIPTION
Complex of human LKB1, also known as STK11 (GenBank Accession No. NM_000455, a.a. 2-433, with N-terminal His-tag), MO25 (GenBank Accession No. NM_016289, a.a. 2-341, with N-terminal GST-tag), and STRAD (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag). Calculated MW=49 kDa* (LKB1), 66 kDa (MO25), and 74 kDa (STRAD), expressed in Sf9 cells using a Baculovirus expression system. *LKB1 runs anomalously on SDS-PAGE.