Recombinant Mouse TNF-alpha Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18 kDa.
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The purity of Mouse TNF-alpha Protein (Cat.RP01071) was greater than 95% as determined by SEC-HPLC.
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Recombinant Mouse TNF-α protein was measured by NF-κB reporter assay in HEK293 cells .The ED<sub>50</sub> for this effect is 40-200 pg/mL.
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Immobilized recombinant Mouse TNFα at 2 μg/mL (100 μL/well) can bind Human TNFRSF1A with a linear range of 4-53 ng/mL.
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Immobilized Recombinant Mouse TNF-α at 2 μg/mL (100 μL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL.
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Recombinant Mouse TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 0.025-0.1 pg/mL.
DETAILS
Tag:
C-His
Source:
HEK293 cells
Gene Id:
21926
Species:
Mouse
Lead Time:
3-5 weekdays
Swiss Prot:
P06804
Research Areas:
TNF family,Cell Culture related
Alternative Names:
DIF, Tnfa, TNF-a, TNFSF2, Tnlg1f, Tnfsf1a, TNFalpha, TNF-alpha,TNF