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51007

MagVigen™ - anti Flag, mouse

NVIGEN

DESCRIPTION

Product Description MagVigen™ – Anti-FLAG nanoparticles are ideal for FLAG-tagged protein purification and immunoprecipitation assays. MagVigen™ Anti-FLAG recognizes and efficiently binds to FLAG-tagged molecules following a short incubation. The generated nanoparticle-FLAG-tag molecule complex can be separated from the rest of the sample by magnet. The retained antibody can be eluted from the nanoparticles using an elution buffer. MagVigen™ nanoparticles enable identification of new protein-protein interactions through immunoprecipitation assays, where the MagVigen™ anti-FLAG FLAG tagged molecule complex can be used to isolate particular proteins of interest or protein complex from assay samples, e.g. cell lysate. The immunoprecipitated proteins can be further analyzed by electrophoresis, protein staining, and mass spectrometry. MagVigen™ nanoparticles are much smaller than conventional micro-beads. This feature allows for better accessibility of the nanoparticles to the antigenic epitope and for less disturbance to the native functions of proteins or protein-protein complexes. In addition, the surfaces of MagVigen™ nanoparticles are uniquely coated to reduce non-specific interactions with cellular proteins and other biomolecules. This feature allows for a more specific “pull down” of real protein complex targets.

DETAILS

  • Category: Purification Reagent
  • Lead Time: 1 week
  • Subcategory: Magnetic Beads
  • Applications: Protein Purification, Co-immunoprecipitation
  • Product and Related Product Contents: Cat# 51007: MagVigen™ – anti-His-Tag nanoparticles (Cat # 51009) are provided in phosphate buffered saline (PBS), pH 7.4. Each vial contains 1 ml of solution with a particle concentration of 1 mg/ml, which is enough for approximately 20-200 antibody enrichment or immunoprecipitation assays. All materials should be stored at 4°C. Shelf life: 6 months