Anti-Actin (N-terminal region) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: ACTB
Host: Rabbit
Rrid: AB_2223214
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Actin (N-terminal region)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Clonality: Rabbit Polyclonal
Immunogen: Actin synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human β actin. This sequence is identical to similar regions in all four α actins, as well as in γ actin, and is well conserved in actins from most eukaryotic species.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of human A431, SYF, and HUVEC cells, as well as mouse C2C12 cells.
Applications: WB, ICC, IHC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 42
Dilution Range Ip: 1:25
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:50
Dilution Range Ihc: 1:100
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.
Uniprot Number Immunogen Species: P60709
Post Translational Modification Residue: 49-62