DESCRIPTION

• Aa: full length
• Mw: 73 kDa
• Tags: N-terminal GST-tag, C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_002093
• Species: Human
• Uniprot: P49841
• Shiptemp: -80°C (dry ice)
• Synonyms: GSK3b, glycogen synthase kinase 3 beta, GSK3beta
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• References: 1. Sutherland C. et al. Biochem J. 1993 Nov 15;296 (Pt 1):15-9. 2. Sperber BR et al. Neurosci Lett. 1995 Sep 8;197(2):149-53. Application Reference(s): 1.Glycogen synthase kinase-3β inhibition in the medial prefrontal cortex mediates paradoxical amphetamine action in a mouse model of ADHD (2015) 2.Induction of Gsk3β-β-TrCP interaction is required for late phase stabilization of β-catenin in canonical Wnt signaling (2014) 3. Leucine-rich repeat kinase 2 regulates tau phosphorylation through direct activation of glycogen synthase kinase-3β (2014)
• Description: Human GSK3β GenBank Accession No. NM_002093), full length, with N-terminal GST-tag and C-terminal His-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Enzyme reactions include 50 µl volumes of 1x Kinase buffer (40 mM Tris_x000D_ pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA)_x000D_ along with 20 µM ATP, 0.2 mg/mL GSKtide_x000D_ substrate, 3mM DTT, and GSTGSK3_x000D_. After 45 min incubation at_x000D_30°C, 50µL of the Kinase-Glo reagent_x000D_ solution was added and luminescence_x000D_ from remaining ATP was measured._x000D_
• Related Products: 40006, 79698, 79700, 78487
• Specific Activity: 391 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine