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11039-H08H

Human N-Cadherin / CD325 / CDH2D Protein (His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the extracellular domain of human CDH2 (NP_001783.2) precursor (Met 1-Ala 724) was fused with a polyhistidine tag at the C-terminus.

DETAILS

  • Tag: C-His
  • Purity: > 90 % as determined by SDS-PAGE
  • Source: HEK293 Cells
  • Species: Human
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 104 cells/well are added to CDH2 coated plates (10 μg/mL with 100 μL/well), approximately 60% will adhere after 1 hour at 37℃.
  • Molecule: CDH2
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4
  • Molecular Mass: The pro form of human CDH2 consists of 710 amino acids and predictes a molecular mass of 78.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of CDH2 is 90 & 75 kDa corresponding to the pro fom and mature form respectinely due to glycosylation.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: NP_001783.2
  • Predicted N Terminal: Ser 26