Nocodazole

DESCRIPTION

Nocodazole is an antineoplastic microtubule polymerization inhibitor. It is frequently used to synchronize cell cycling and arrest cells with a G2- or M- phase DNA content. It is reported to enhance homology-directed repair (HDR) efficiency and increase Cas9-mediated editing frequencies.

DETAILS

Purity: ≥98%
Synonyms: Nocodazolum, ChEMBL9514, Oncodazole
Cas Number: 31430-18-9
References: Vasquez, R. J., Howell, B., Yvon, A. M., Wadsworth, P., & Cassimeris, L. (1997). Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Molecular biology of the cell, 8(6), 973-985. Yang, D., Scavuzzo, M. A., Chmielowiec, J., Sharp, R., Bajic, A., & Borowiak, M. (2016). Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Scientific reports, 6. Lin, S., Staahl, B. T., Alla, R. K., & Doudna, J. A. (2014). Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife, 3, e04766.
Application: FA
Formulation: Crystalline solid
Chemical Name: methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate
Molecular Weight: 301.3
Molecular Formula: C14H11N3O3S
Storage Conditions: Product should be kept at -20°C.
Pubchem Openeye Can Smiles: COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3