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11556-H20B

Human PTPN12 Protein (aa 1-355, His & GST Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

DETAILS

  • Tag: N-GST & His
  • Purity: > 90 % as determined by SDS-PAGE
  • Source: Baculovirus-Insect Cells
  • Species: Human
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate, R&D Systems, Catalog # ES006. The specific activity is > 15 μmoles/min/mg.
  • Molecule: PTPN12
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0.
  • Molecular Mass: The recombinant human PTPN12 chimera consists of 592 amino acids and has a calculated molecular mass of 69.4 kDa. The recombinant protein migrates approximately 64 kDa band in SDS-PAGE under reducing conditions.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: AAA36529.1
  • Predicted N Terminal: Met