DESCRIPTION

• Tag: C-His
• Form: Lyophilized
• Type: Recombinant Protein
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Target: Envelope glycoprotein E
• Species: Varicella-zoster virus (strain Oka vaccine)
• Shipping: Shipped at ambient temperature.
• Synonyms: Envelope glycoprotein E; gE; ORF68;
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Background: VZV glycoprotein E (gE) is most abundantly expressed on the surface of infected cells, and is an essential component for virus replication and cell-to-cell transmission. It is also the main target of virus-specific antibodies and T cell responses that is often selected as vaccine candidate antigen.
• Description: Recombinant Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Ser31-Tyr538.
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Biotinylated: no
• Exact Sequence: Ser31-Tyr538
• Express System: HEK293
• Geneprotein Id: Q9J3M8
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Molecular Weight: The protein has a predicted MW of 58.39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.
• Stability and Storage: -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.