79900
UBCH5a TR-FRET Assay Kit
BPS Bioscience
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DESCRIPTION
UBCH5a (UBE2D1) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5a TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5a protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
DETAILS
- Shiptemp: -80°C (dry ice)
- Warnings: Avoid freeze/thaw cycles.
- Category: Ubiquitin/assay kit
- References: 1. Saville, M.K., et al., J. Biol. Chem. 2004; 279(40): 42169-81.2. Kim, J.-H., et al., BMB Rep. 2015; 48(1): 25-29.
- Description: UBCH5a (UBE2D1) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5a TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5a protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
- Methodology: TR-FRET
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Useful for screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 80315, 79741, 79896, 79901
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Ubiquitination
- Instructions for Use: See assay protocol for detailed instructions.